Couples who have been unsuccessfully trying to have a baby for long periods of time can find the initial phases of the IVF process quite overwhelming. Our center ensure that the couple has all the clinical and scientific information, emotional support and administrative convenience that they may require for starting their journey, irrespective of whether it culminates in a pregnancy.
Gaudium IVF- The center makes use of several medications, along with hormones, which induce the ovaries to give rise to many natural and normal follicles.
This may result in the harvesting of multiple oocytes and thus the development of multiple embryos. The excess may be cryopreserved, to be used if the couple plan to have more children in the future.
The development of the ovarian follicles is closely observed by measuring blood hormones levels and also by visualizing the ovaries by means of ultrasounds. The most commonly assessed hormones are FSH, LH and estradiol. Ultrasounds are performed multiple times to make sure that the size and dimensions of the ovarian follicle are optimum.
This is done of that the team of experts is in fully aware of the ovarian response; if in case the response is not as good as expected, the process may be stopped and continued in the next cycle. Upon satisfactory folliculogenesis, the female receives a shot of the hormone hCG (human chorionic gonadotropin), which aids the development of the oocyte and ovulation. These mature oocytes are then harvested. The team may withhold the hCG and wait for natural and spontaneous ovulation. This caused by the LH surge after estrogen levels are at their peak.
Once the blood work and ultrasounds indicate the correct development of the oocytes, they are harvested form the uterus. This process involves the patient undergoing general anesthesia for about 30 minutes. The state of the art facility at the Gaudium IVF is then used to carefully locate each of the follicles and then retrieve them carefully through trans-vaginal aspiration. The aspirate is then immediately rushed to the IVF laboratory. Patients take a few hours to recover from the anesthesia and are then sent back with progesterone supplements.
The laboratory at the Gaudium IVF Center first examines all the follicles for well-developed oocytes. These are retrieved and placed in a culture medium, where sperms are introduced next. The semen is usually collected at the time of oocyte harvest, but this process may be delayed to ensure freshness. Once the egg are fertilized, they cleave and form embryos in the culture medium. These embryos are then either implanted or cryopreserved.
It takes about 2 to 3 days for the embryos to develop post harvesting, after which they are evaluated for their size and structure under a microscope. The healthy ones are loaded meticulously in a catheter and the contents are released in the uterus per-vaginally. This step does not need anesthesia, but a mild sedative may be administered to ensure cervical relaxation. Only 3 – 4 embryos are used in this step.
The patient is asked to rest for 2 – 3 hours post the procedure to assist implantation, after which she is discharged. The patient is recommended bed rest for 4 days and is called back in for hormonal assay on day 6. On day 12, a serum pregnancy test is conducted. The patient is advised mild activity in these days and is requested to be in touch with the center for dispelling any doubts.
Those embryos that are viable but are not implanted can be cryopreserved. The experts at Gaudium IVF assess each of the remnant embryos for suitability. Those that have uniform blastomeric cells with minimal or zero fragmentation are selected for the same.